M. Kapun, M.G. Barrón, F. Staubach, D.J. Obbard, R.A.W. Wiberg, J. Vieira, C. Goubert, O. Rota-Stabelli, M. Kankare, M. Bogaerts-Márquez, A. Haudry, L. Waidele, I. Kozeretska, E.G. Pasyukova, V. Loeschcke, M. Pascual, C.P. Vieira, S. Serga, C. Montchamp-Moreau, J. Abbott, P. Gibert, D. Porcelli, N. Posnien, A. Sánchez-Gracia, S. Grath, É. Sucena, A.O. Bergland, M.P.G. Guerreiro, B.S. Önder, E. Argyridou, L. Guio, M.F. Schou, B. Deplancke, C. Vieira, M.G. Ritchie, B.J. Zwaan, E. Tauber, D.J. Orengo, E. Puerma, M. Aguadé, P.S. Schmidt, J. Parsch, A.J. Betancourt, T. Flatt, J. González, Genomic Analysis of European Drosophila melanogaster Populations Reveals Longitudinal Structure, Continent-Wide Selection, and Previously Unknown DNA Viruses, Molecular Biology and Evolution, Volume 37, Issue 9, Pages 2661–2678, 2020.
Abstract: Genetic variation is the fuel of evolution, with standing genetic variation especially important for short-term evolution and local adaptation. To date, studies of spatiotemporal patterns of genetic variation in natural populations have been challenging, as comprehensive sampling is logistically difficult, and sequencing of entire populations costly. Here, we address these issues using a collaborative approach, sequencing 48 pooled population samples from 32 locations, and perform the first continent-wide genomic analysis of genetic variation in European Drosophila melanogaster. Our analyses uncover longitudinal population structure, provide evidence for continent-wide selective sweeps, identify candidate genes for local climate adaptation, and document clines in chromosomal inversion and transposable element frequencies. We also characterize variation among populations in the composition of the fly microbiome, and identify five new DNA viruses in our samples.
Keywords: Population genomics, adaptation, demography, selection, clines, SNPs, structural variants
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Keywords: Population genomics, adaptation, demography, selection, clines, SNPs, structural variants
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Ayhan, N., Güler, P., Önder, B.S., Lipocalin genes expression varied in developmental stages by larval nutritional stress in Drosophila, Turkish Journal of Biology, 41: 178-186, 2017.
Abstract: Lipocalin genes NLaz, GLaz, and Karl are evolutionarily conserved genes in Drosophila melanogaster. There are studies on lipocalin gene expression differences under diverse diet conditions, but these studies have focused mainly on age-dependent expression profiles of these genes. The main aim of our study is to determine lipocalin expression in the developmental period by nutritional manipulation with an isofemale-based design. Three larval developmental periods have been researched under normal and restricted diets. We found significant differences between lines during their developmental time-related lipocalin expression. Here, we demonstrate that upregulations in the early developmental stages of lipocalin genes under stressful conditions resulted in unaffected developmental time. The possible reason for high expression is the activation of stress signal pathways in order to buffer the harmful effects of nutritional restriction. Our data showed that the early developmental period (48-72 h) is especially crucial to tolerate the dietary stress with respect to GLaz and NLaz expression. Results of this experiment have shown that the expression profiles of lipocalin genes have line-specific pathways to nutritional stress. Their expression depends on the genetic background corresponding to development time results. Our results highlight the transcriptional changes of lipocalins associated with developmental time in larvae, developed in a dietary-restricted medium.
Keywords: Dietary stress, lipocalin gene family, ApoD, developmental time, Drosophila melanogaster.
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Keywords: Dietary stress, lipocalin gene family, ApoD, developmental time, Drosophila melanogaster.